The University Chorus is comprised of voice, piano, guitar, composition, and music therapy majors, as well as non-music majors. The ensemble performs a wide variety of a cappella and accompanied choral repertoire. The ensemble performs at least two concerts each semester, and frequently performs with instrumental ensembles.

Singing Cardsmen is the premier men's chorus of the School the Music. The Cardsmen was established in 2015-2016 in efforts to extend the choral experiences to more students at the University of Louisville. This non-auditioned ensemble provides an opportunity for the men to act as ambassadors, representing students from all walks of life in the university.

The Cardinal Singers, directed by Dr. Kent Hatteberg, international festival appearances include the U.S./Cuba Choral Symposium in Havana, Cuba in 2012, the Taipei (Taiwan) and Beijing (China) International Choral Festivals in 2010, and the 7th World Symposium on Choral Music in Kyoto, Japan in 2005. They won the Grand Prize at the Yeosu (Korea) International Choir Competition and the H?i An Choir Prize at the 3rd Vietnam International Choir Competition in 2013.