Collegiate Chorale, directed by Dr. Kent Hatteberg, is the premier choral ensemble of the School of Music. The Chorale performed at the 2011 National ACDA Convention in Chicago, in the feature major works concerts at the 2009 ACDA National Convention in Oklahoma City, in the Headliner Concert at the 2008 ACDA Southern Division Convention in Louisville, and at the 2005 ACDA National Convention in Los Angeles. Although the Chorale performs primarily a cappella repertoire, they collaborate regularly with the Louisville Orchestra and Orchestra Kentucky in the performance of major works.

The University Chorus is comprised of voice, piano, guitar, composition, and music therapy majors, as well as non-music majors. The ensemble performs a wide variety of a cappella and accompanied choral repertoire. The ensemble performs at least two concerts each semester, and frequently performs with instrumental ensembles.

Singing Cardsmen, directed and founded by Dr. Randi Bolding, is the premier men's chorus of the School the Music. The Cardsmen was established in 2015-2016 in efforts to extend the choral experiences to more students at the University of Louisville. This non-auditioned ensemble provides an opportunity for the men to act as ambassadors, representing students from all walks of life in the university.